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Microwave-assisted Eco-Friendly Synthesis and Antimicrobial Evaluation of Aryl-Triazole-1,3,4-Thiadiazols

Shalini Jaiswal, Smriti Dwivedi


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Due to the growing awareness about environmental pollution and environmental legislation, recent years have witnessed a phenomenal increase in the application of microwave irradiation (MW) in organic synthesis. Heterocyclic compounds are abundant in nature and are of great significance to life because their structural subunits exist in many natural products such as vitamins, hormones, and antibiotics; hence, they have attracted considerable attention in the design of biologically active molecules and advanced organic chemistry. The application of molecular diversity technique to drug discovery is a multidisciplinary effort in organic synthesis. Medicinal chemistry concerns with the discovery, development, interpretation and the identification of mechanism of action of biologically active compounds at the molecular level. Encouraged by above reports and as part of our research programme for development of eco-friendly synthetic protocol for biologically active compounds as well as in pursuing of our work on new solvent-free cyclisation here we report the synthesis of aryl-triazalo -1, 3, 4-thidiazoles. The one-pot reaction of mercapto-s-triazole with aromatic acid using AlCl3 as a catalyst under microwave irradiation (2-3 min) and in solvent-free condition gave aryl-triazalo -1, 3, 4-thidiazoles with improved yield is described here.

Keywords: Aryl-triazalo-1, 3, 4-thiadiazoles, Aromatic acid, AlCl3, cyclisation, S. aureus, E. coli, B. subtilis.


Heterocyclic compounds are cyclic compounds in which one or more of the atoms of the ring are heteroatom. A heteroatom is an atom other then carbon. The heterocyclic ring comprises the core of the active moiety or pharmacophore. An especially big attention is given to sulphur and nitrogen containing heterocyclic compounds, as they possess a broad spectrum of biological activity [1]. Heterocyclic compounds have attracted considerable attention in the design of biologically active molecules [2,3] and advanced organic chemistry [4].

Heterocyclic compounds containing two and three nitrogen in its ring structure i.e. Pyrazoles, imidazole and triazole are an important class of hetero aromatic systems. The traditional method available for the synthesis of title compound is not satisfactory because it requires long reaction times, use of expensive and hazardous solvents and yield are only moderates. Due to the growing awareness about environmental pollution and environmental legislation, recent years have witnessed a phenomenal increase in the application of microwave irradiation (MW) in organic synthesis [5, 6, 7]. Due to its operational simplicity, generality and efficiency this method is expected to have wider applicability in organic synthesis.

The synthesis of nitrogen containing heterocyclic compound has increasing interest over the past decade because of their utility in various applications, such as propellants, explosives, pyrotechnics and especially chemotherapy. In recent years, the chemistry of triazole and their fused heterocyclic derivatives has received considerable attention owing to their synthetic and effective biological importance [8].

The triazole nucleus has been synthesized as an antiviral agent for inhibiting the metastasis of malignant tumor cell. The fusion of two or more heterocyclic nuclei often enhance biological profile may fold than its parent nuclei. The Nitrogen bridged heterocycle developed from triazole possesses anti-inflammatory [9], antifungal [10, 11] herbicidal [12], antimicrobial [13], antiparasitic [14], cytostatic [15], brassinosteroid biosynthesis inhibitory activities [16] activities.

The triazole nucleus is one of the most important heterocycle which is a feature of natural products and medicinal agents. The derivatization of Triazole ring is based on the phenomenon of bioisosterism in which replacement of oxygen of oxadiazole nucleus with nitrogen triazole analogue. These modifications are carried out by using different functionalities, aliphatic chains, aromatic rings and heterocyclic ring systems [17, 18, 19, 20].

The one-pot reaction of mercapto-s-triazole 1 with aromatic acid 2 using AlCl3 as a catalyst under microwave irradiation (2-3 min) and in solvent-free condition gave aryl-triazole -1, 3, 4-thidiazoles 3a-e with improved yield is mentioned in this paper.

Materials and Methods

Experimental Section

Melting points were determined by open glass capillary method. All chemicals used were reagent grade and were used as received. A Laboratory Microwave Oven (Model BP 310/50) operating at 2450 MHz and power output of 600 W was used for all the experiments. The completion of reactions was monitored by TLC (Merck silica gel). IR spectra were recorded on a Shimadzu FTIR-420 spectrophotometer. 1H NMR and 13C NMR spectra were recorded at 400oC on a Bruker AVANCE DPX (400 MHz) FT spectrometer in CDCl3 using TMS as an internal reference (chemical shift in δ, ppm). Mass spectra were recorded on JEOL SX-303 (FAB) mass spectrophotometer at 70ev. Elemental analyses were carried out using a Coleman automatic C, H, N analyzer.


5-Aryl-4-amino-3-mercapto-s-triazale (.02 mol), aromatic acid 2 (.02 mol) and catalytic amount of AlCl3 were mixed thoroughly in a beaker and irradiate in microwave at 500 w for specified period (1-3 min). The reaction-mixture was cooled and poured on crushed ice. The solid separated was filtered, treated with dil. KOH, washed with water and crystallized from Benzene to obtained 3 (a-f).

3-(4-Aminophenyl)-5-Phenyl-s-triazalo-[3, 4-5] 1, 3, 4-thiadiazole (3a)

IR (KBr) (Cm-1); 3275 (NH2), 3080 (aromatic C-H stretching) ,1HNMR-(CDCl3); 77-8.1 (9H, m, ArH), 5.9 (*2H, S, NH2) M.P. = 208oC, yield = 88%

3-(4-aminophenyl)-5-(4-aminophenyl)-s-triazalo-3,4-b]-1, 3, 4-thiadiazole (3b)

IR (KBr) (Cm-1); 3275 (N-H), 3080 (aromatic C-H stretching) ‘1HNMR-(CDCl3); 7.9-8.4 (8H, m, ArH), 6.01 (4H, S, NH2) M.P. = 233 oC, yield = 89%

3-(4-Aminophenyl)-5-(4-nitrophenyl)-s-triazalo-[3, 4-b]-1, 3, 4-thiadiazole (3c)

IR (KBr) (Cm-1); 3275 (N-H), 3080 (aromatic C-H stretching) ‘1HNMR-(CDCl3); 7.9-8.4 (8H, m, ArH), 6.0 (4H, S, NH2) M.P. = 238 oC, yield = 87%

3-(4-Aminophenyl)-5-(4-chlorophenyl)-s-triazalo-[3, 4-b]-1, 3, 4-thiadiazole (3d)

IR (KBr) (Cm-1); 3275 (N-H), 3080 (aromatic C-H stretching) ‘1HNMR-(CDCl3); 8.0-8.6 (8H, m, ArH), 5.9 (2H, S, NH2) M.P. = 230 oC, yield = 92%

3-(4-Aminophenyl)-5-(4-hydroxyphenyl)-s-triazalo-[3, 4-b]-1, 3, 4-thiadiazole (3e)

IR (KBr) (Cm-1); 3275 (N-H), 3080 (aromatic C-H stretching) ‘1HNMR-(CDCl3); 8.0-8.6 (8H, m, ArH), 5.9 (2H, S, NH2) M.P. = 224 oC, yield = 90%

3-(4-Aminophenyl)-5-(4-methoxyphenyl)-s-triazalo-[3, 4-b]-1, 3, 4-thiadiazole (3f)

IR (KBr) (Cm-1); 3275 (N-H), 3080 (aromatic C-H stretching) ‘1HNMR-(CDCl3); 7.0-8.1 (8H, m, ArH), 5.9 (2H, S, NH2), 4.01 (3H, S, OCH3) M.P. = 242 oC, yield = 89%

Antimicrobial screening

The antibacterial activity of the synthesized heterocyclic compounds 3a-f was tested against both Gram-negative and Gram-positive bacteria. The test solutions were prepared in benzene. The inhibition zone against the growth of the verified bacteria for the compounds is given in Table 1. It seems that enhanced biological activity for the compound 3b, 3e and 3f is due to its electron donating group (hydroxyl, methoxy and amino) and the poly-conjugated nature of the compound.

Table 1 Antibacterial Activities of compounds 3a-f
Compd. S. aureus E. coli B. subtilis
3a 7 8 7
3b 13 13 14
3c 11 12 11
3d 12 12 10
3e 13 13 11
3f 12 14 12
Ciprofloxacin 15 16 -
Nystatin - - 13

Results and Discussion

The conventional method available for the title compound is not satisfactory because it requires long reaction times, use of expensive and hazardous solvents and yield are only moderates.

The reactions were also carried out using a thermostated oil-bath at the same temperature (120oC) as for the MW activated method but for a longer period of time. It was found that MW method has improved the yields significantly. This observation can be rationalized on the basis of the formation of dipolar activated complex from uncharged adduct in these reactions and greater stabilization of the more dipolar activated complex by dipole-dipole interaction with electric field of the microwaves as compared to the less polar adduct which may reduce the activation energy resulting in the rate enhancement.

The ortho-, meta- or Para-position of the electron donating groups in the aromatic system plays a significant role in the bioactivities. In addition to this, the presence of heteroatom such as oxygen, sulphur, and nitrogen also play a vital role in the observed antibacterial activity.It is also suggested that the sulphur-containing compounds might inhibit enzyme synthesis, since enzymes need specific groups for their activity and are especially susceptible to deactivation by the compounds. The presence of sulphur and nitrogen atoms in the structure of the active compounds facilitates their diffusion through the lipid layer of the microorganism membranes to the site of action, eventually killing them by linking with essential groups of certain cell enzymes


The major challenges for medicinal chemistry the development of simple, effective and eco- friendly method for the synthesis of heterocyclic compound with enhance antimicrobial activity.

Now a day various methods have been proposed for the preparation of triazalo and thiadiazols derivative but, all of this reported method has several drawbacks like ,use of organic solvents which causes, pollution, strong reaction conditions, time consuming reactions and use of expensive reagents. To solve such type of problems here we report a simple, practically feasible and eco-friendly method for the synthesis of triazalo -1, 3, 4-thidiazoles derivatives. The growth of green chemistry is important because it has many advantages like, short reaction time, eco-friendly, pollution free and use of energy. In view of importance of triazalo and thiadiazols derivatives as a chemotherapeutic agent, and development of a facile, efficient and environmentally benign method to synthesize this heterocyclic compound would be great value. This observation prompted us to develop an environment friendly approach for synthesis of substituted triazalo -1, 3, 4-thidiazoles which possesses various antimicrobial activities.


Authors are thankful to head, Department of Chemistry, AMITY University Greater Noida and RSIC, CDRI Lucknow for spectral analysis.


  1. Lakshmi PC, Esther RV, Spoorthy YN, Ravindranath LK. Synthesis characterization and antimicrobial activity of 6-nitro-1H-benzo[d]imidazole-2-yl) methyl)-6-oxido-4,8-dihydro1H-[1, 3, 2] dioxaphosphepino [5,6-c] pyrazole-6-yl) ureas/carboxamides-Mannich bases. Journal of Chemical and Pharma ceutical Research. 2013;5:280-. View Article Google Scholar
  2. Lokhande P, Waghamare B, Sakate S. Regioselective one-pot synthesis of 3, 5-diarylpyrazoles. Indian journal of chemistry. 2005;44(11):2338-42. View Article Google Scholar
  3. Reddy GJ, Manjula D, Rao KS. A Direct Single Step Synthesis of 1, 3‐Diaryl‐4‐cyanopyrazoles and Their Conversion to 1, 3‐Diaryl‐4‐(4, 6‐diamino‐1, 3, 5‐triazin‐2‐yl) pyrazoles. ChemInform. 2006;37(11):-. View Article Google Scholar
  4. Zificsak CA, Hlasta DJ. Current methods for the synthesis of 2-substituted azoles. Tetrahedron. 2004;60(41):8991-9016. View Article Google Scholar
  5. Yadav LD, Singh S, Singh A. Novel clay-catalysed cyclisation of salicylaldehyde semicarbazones to 2H-benz [e]-1, 3-oxazin-2-ones under microwave irradiation. Tetrahedron letters. 2002;43(47):8551-3. View Article Google Scholar
  6. Mogilaiah K, Kavitha S, Babu HR. Microwave Assisted Addition—Elimination Reactions of 3‐Phenylimino‐2‐indolinones with 2‐Hydrazino‐3‐(p‐chlorophenyl)‐1, 8‐naphthyridine: A Simple and Facile Synthesis of 3‐(3‐p‐Chlorophenyl‐1, 8‐naphthyridin‐2‐yl hydrazono)‐2‐indolinones. ChemInform 2003 Nov. 4;34(44):-. View Article Google Scholar
  7. McGee DP, Martin JC, Smee DF, Verheyden JP. Synthesis and antiviral activity of the 3-deaza analogue of 9-((1, 3-dihydroxy-2-propoxy) methyl) guanine. Nucleosides, Nucleotides & Nucleic Acids. 1990;9(6):815-26. View Article Google Scholar
  8. El-Serwy WS, Mohamed NA, Abbas EM, Abdel-Rahman RF. Synthesis and anti-inflammatory properties of novel 1, 2, 4-triazole derivatives. Research on Chemical Intermediates. 2013;39(6):2543-2554. View Article Google Scholar
  9. Kocyigit-Kaymakcioglu B, Celen AO, Tabanca N, Ali A, Khan SI, Khan IA, Wedge DE. Synthesis and biological activity of substituted urea and thiourea derivatives containing 1, 2, 4-triazole moieties. Molecules. 2013;18(3):3562-76. View Article Google Scholar
  10. Liu XH, Tan CX, Weng JQ. Synthesis, dimeric crystal structure, and fungicidal activity of 1-(4-methylphenyl)-2-(5-((3, 5-dimethyl-1H-pyrazol-1-yl) methyl)-4-phenyl-4H-1, 2, 4-triazol-3-ylthio) ethanone. Phosphorus, Sulfur, and Silicon. 2011;186(3):558-64. View Article Google Scholar
  11. Tong JY, Wu HK, Sun NB, Liu XH. Synthesis, crystal structure and biological activity of a new 1, 2, 4-triazole derivative. Chin J Struct Chem. 2013;32:607-611. View Article Google Scholar
  12. Ke W, Sun NB. Microwave assistant synthesis, crystal structure and biological activity of a 1, 2, 4-triazole compound. Journal of the Chemical Society of Pakistan. 2013;35(4):1241-6. View Article Google Scholar
  13. Al-Omar MA, Al-Abdullah ES, Shehata IA, Habib EE, Ibrahim TM, El-Emam AA. Synthesis, antimicrobial, and anti-inflammatory activities of novel 5-(1-adamantyl)-4-arylideneamino-3-mercapto-1, 2, 4-triazoles and related derivatives. Molecules. 2010;15(4):2526-50. View Article Google Scholar
  14. Franklim TN, Freire-de-Lima L, de Nazareth Sá Diniz J, Previato JO, Castro RN, Mendonça-Previato L, de Lima ME. Design, synthesis and trypanocidal evaluation of novel 1, 2, 4-triazoles-3-thiones derived from natural piperine. Molecules. 2013;18(6):6366-82. View Article Google Scholar
  15. Benci K, Mandić L, Suhina T, Sedić M, Klobučar M, Kraljević Pavelić S, Pavelić K, Wittine K, Mintas M. Novel coumarin derivatives containing 1, 2, 4-triazole, 4, 5-dicyanoimidazole and purine moieties: Synthesis and evaluation of their cytostatic activity. Molecules. 2012;17(9):11010-25. View Article Google Scholar
  16. Yamada K, Yoshizawa Y, Oh K. Synthesis of 2RS, 4RS-1-[2-phenyl-4-[2-(2-trifluromethoxy-phenoxy)-ethyl]-1, 3-dioxolan-2-yl-methyl]-1H-1, 2, 4-triazole derivatives as potent inhibitors of brassinosteroid biosynthesis. Molecules. 2012;17(4):4460-73. View Article Google Scholar
  17. Calderone V, Fiamingo FL, Amato G, Giorgi I, Livi O, Martelli A, Martinotti E. 1, 2, 3-Triazol-carboxanilides and 1, 2, 3-triazol-(N-benzyl)-carboxamides as BK-potassium channel activators. XII European journal of medicinal chemistry. 2008;43(11):2618-26. View Article Google Scholar
  18. Kim EM, Joung MH, Lee CM, Jeong HJ, Lim ST, Sohn MH, Kim DW. Synthesis of Tc-99m labeled 1, 2, 3-triazole-4-yl c-met binding peptide as a potential c-met receptor kinase positive tumor imaging agent. Bioorganic & medicinal chemistry letters. 2010;20(14):4240-3. View Article Google Scholar
  19. Giffin MJ, Heaslet H, Brik A, Lin YC, Cauvi G, Wong CH, McRee DE, Elder JH, Stout CD, Torbett BE. A copper (I)-catalyzed 1, 2, 3-triazole azide− alkyne click compound is a potent inhibitor of a multidrug-resistant HIV-1 protease variant. Journal of medicinal chemistry. 2008;51(20):6263-70. View Article Google Scholar
  20. Wang Q, Chittaboina S, Barnhill HN. Highlights in organic chemistry advances in 1, 3-dipolar cycloaddition reaction of azides and alkynes-a prototype of “click” chemistry. Letters in Organic Chemistry. 2005;2(4):293-301. View Article Google Scholar
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Article Details

Issue: Vol 1 No 3 (2017)
Page No.: 17-22
Received: Apr 26, 2017
Published: May 26, 2017
Section: Review Article

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Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How to Cite
Jaiswal, S., & Dwivedi, S. (2017). Microwave-assisted Eco-Friendly Synthesis and Antimicrobial Evaluation of Aryl-Triazole-1,3,4-Thiadiazols. Journal Of Medical Research And Innovation, 1(3), 17-22. doi:10.5281/zenodo.583601
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